Coding

Part:BBa_K108015:Experience

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)


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Applications of BBa_K108015

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Edinburgh OG 2018

We access the online NCBI data and found out the current available phaR sequence (AM260479.1) is being 4 bp different to BBa_K108015. Therefore we have used the new phaR and promoter sequence as our new template. Due to the excessive GC in the sequence, we have it codon optimised to E.coli and generated the part through the IDT company service.


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Figure 1. Comparison of phaR sequence from iGEM08_Tsinghua and online NCBI. Capital letters: NCBI and Small letter: iGEM08_Tsinghua.

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Figure 2. Comparison of phaR sequence before and after codon optimisation. Capital letters: NCBI and Small letter: codon optimised version.

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User Reviews

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